The Definitive Guide to cytometers

The Definitive Guide to cytometers

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Information-enriched characterization of cell populations. Morphological info from images can incorporate to your richness of flow cytometry data. One example is, the determine exhibits an otherwise conventional apoptosis assay applying Annexin V and PI, including cell imaging to characterize cells in Just about every population to expose morphologically distinctive functions. These insights couldn't have already been received from multiplex staining on your own.

No matter if you’re a beginner or an authority, explore our sources to progress your study and experience in flow cytometry.

Mix acoustic focusing engineering with higher-parameter Assessment for versatile spectral unmixing and traditional workflows.

Learn new insights into your experimental info by visualizing populations employing mixed fluorescence and morphology details. Our software package interface wizards permit downsampling, concatenation (merged documents) and dimensionality resources which have been built in the application for an intuitive, streamlined information Evaluation experience.

"An image just isn't really worth a thousand dots, but it really seriously helps to see images. It is great to find out photographs that correlate with staining. For instance, AnnexinV-positive cells that are morphologically various than healthy cells, or CD14+ monocytes that are greater and a lot more textured than CD3+/CD4+/CD14- lymphocytes.

Listed here, a qualified consumer has gated singlets confidently. Just after evaluating the handbook singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate has much more than four% aggregates.

Pair your Attune flow cytometer using an autosampler for elevated workflow effectiveness, and increase robotic integration for max throughput.

Consistent picture top quality even at high throughput Acoustic focusing along with a significant-velocity digital camera Merge to image these T cells persistently at low or superior flow prices. Effortlessly modify aim and camera configurations to fulfill experimental necessities.

The Attune NxT cytometer is the trustworthy workhorse instrument your lab can depend on for regular and reliable performance. The Attune CytPix design encompasses a large-velocity brightfield camera and AI-driven graphic Examination software program, with latest upgrades which includes equipment Understanding equipment and an intuitive user interface, enabling Increased information precision, precision, and more rapidly insights into sample biology.

Possibly most of all, these activities contain cells of Obviously distinct phenotypes which could lead on to incorrect conclusions pertaining to double beneficial gatherings (specifically in scarce populations).

Seem waves empower specific mobile alignment, leading to Excellent details high-quality and ~10X a lot quicker sample flow rates.

We Formerly demonstrated the power of imaging Automobile-T/Ramos mobile interactions. Enable’s check out just the population of finest interest, the double beneficial functions, to learn more. We can easily now use extended morphological parameters (below, circularity and skewness of intensity) to further more take a look at the functions of these populations and refine gating on these occasions, increasing knowledge robustness.

Created-in dimensionality reduction instruments increased resolution into populations. Fluorescently gated populations again-gated on to UMAP plot to confirm predicted populace resolution in lysed whole human blood. User can view occasions from Every cluster for Visible confirmation of cell phenotypes.

No clean/no lyse leukocyte analysis with imaging. Cells had been obtained from 24-hour-aged blood by dilution in one mM EDTA ( Professional UX/UI permits consumers to teach AI types for expanded purposes. (A) The “Train Model” feature presents annotation applications and actual-time design education accuracy.

Gating on these extended parameters lets you quickly and precisely discover populations of curiosity and make sure gating tactic with little or no guide critique. The Attune software program provides entry to point out-of-the-art machine Finding out Examination resources within just an expertly created user interface, which makes it extremely website convenient to use.

Because the mobile line had previously been analyzed around the Attune CytPix Flow Cytometer, the researchers went back to the images and were capable to document the microbial infection not less than 5 days before.

Agilent Benefit Promise ensures you at the very least a decade of instrument use within the day you buy. In any other case, We are going to credit score you While using the residual worth of the process toward an upgraded product.

What seemed to be a homogeneous populace is really extra diverse—an Perception that should be considered when interpreting outcomes.